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A centuries old problem in nephtheid taxonomy approached using DNA data (Coelenterata: Alcyonacea) Naturalis
Ofwegen, L.P. van; Groenenberg, D.S.J..
The current centuries old classification of the family Nephtheidae is still mostly based on colony morphology. In this family the Indo-Pacifc genera Litophyton, Nephthea, Dendronephthya and Stereonephthya, and the Atlantic genus Neospongodes form a complex mix of closely related, poorly described species which cannot be recognized using only colony morphology. Attempts with the more modern approach of comparing skeleton composition (sclerites) have been only partly successful because of the extreme variation of sclerite forms present in these genera. The genus Chromonephthea Van Ofwegen, 2005, introduced for several species previously assigned to Dendronephthya, Nephthea and Stereonephthya, was established with sclerite morphology, but the true generic...
Tipo: Article / Letter to the editor Palavras-chave: Soft corals; Octocorallia; Nephtheidae; Dendronephthya; Litophyton; Neospongodes; Nephthea; Stereonephthya; Taxonomy; Internal transcribed spacer I and II; Indo-Pacific; 42.72.
Ano: 2007 URL: http://www.repository.naturalis.nl/record/256122
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DNA barcoding of the leaf-mining moth subgenus Ectoedemia s. str. (Lepidoptera: Nepticulidae) with COI and EF1-α: two are better than one in recognising cryptic species Naturalis
Nieukerken, E.J.; Doorenweerd, C.; Stokvis, F.R.; Groenenberg, D.S.J..
We sequenced 665bp of the Cytochrome C Oxidase I (COI) barcoding marker for 257 specimens and 482bp of Elongation Factor 1-α (EF1-α) for 237 specimens belonging to the leafmining subgenus Ectoedemia (Ectoedemia) in the basal Lepidopteran family Nepticulidae. The dataset includes 45 out of 48 West Palearctic Ectoedemia s. str. species and several species from Africa, North America and Asia. Both COI and EF1-α proved reliable as an alternative to conventional species identification for the majority of species and the combination of both markers can aid in species validation. A clear barcode gap is not present, and in some species large K2P intraspecific pairwise differences are found, up to 6.85% in COI and 2.9% in EF1-α. In the Ectoedemia rubivora species...
Tipo: Article / Letter to the editor Palavras-chave: Pairwise difference; Palearctic; 42.75; 42.64.
Ano: 2012 URL: http://www.repository.naturalis.nl/record/443278
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On the identity of broad-shelled mussels (Mollusca, Bivalvia, Mytilus) from the Dutch delta region Naturalis
Groenenberg, D.S.J.; Wesselingh, F.P.; Rajagopal, S.; Jansen, J.M.; Bos, M.M.; Velde, G. van der; Gittenberger, E.; Hoeksema, B.W.; Raad, H.; Hummel, H..
Late Quaternary (Eemian) deposits of the Netherlands contain shells that resemble those of living Mytilus galloprovincialis. Similar broad-shelled mytilids also occur in estuaries of the southwestern Netherlands together with slender individuals typical of M. edulis. We sampled living mussels along a depth gradient in the Oosterschelde to a) investigate whether a relation exists between shell shape and depth, b) test if the broadshelled specimens might represent M. galloprovincialis (or a hybrid with M. edulis) and c) assess by inference if the Quaternary specimens might be attributed to M. galloprovincialis as well. In order to do so, we compared genetic (length polymorphism of Me 15/16, COIII sequences and AFLPs) and shellmorphological characteristics...
Tipo: Article / Letter to the editor Palavras-chave: AFLP; COIII; Me 15/16; Mytilus; Shell morphology; The Netherlands; 42.73.
Ano: 2011 URL: http://www.repository.naturalis.nl/record/376595
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Systematics of Ariantinae (Gastropoda, Pulmonata, Helicidae), a new approach to an old problem Naturalis
Groenenberg, D.S.J.; Subai, P.; Gittenberger, E..
A new starting-point in Ariantinae systematics is presented by combining data on traditional shell morphology and genital anatomy, with phylogeny reconstructions based on DNA sequence data. For nearly all genera and subgenera one or more shells are depicted and drawings of the proximal part of the genital organs are shown to illustrate the morphological diversification within the subfamily. For as much as our material allowed it, partial sequences are presented for Histone H3 (H3), Cytochrome c oxidase subunit I (COI), Cytochrome B (CytB) and 16S ribosomal RNA (16S). Some of the allegedly speciose genera like Chilostoma and Campylaea (Zilch, 1960) do not represent monophyletic groups of species, whereas most of the remaining nominal taxa (e.g. Causa,...
Tipo: Article / Letter to the editor Palavras-chave: Ariantinae; Classification; Molecular phylogeny; Morphology.
Ano: 2016 URL: http://www.repository.naturalis.nl/record/607662
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